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Biology 1967

On the Origin of Mitosing Cells

Lynn Sagan (Lynn Margulis)

Your cells' power plants were once free-living bacteria — swallowed, kept, and never let go.

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In depth · the introduction

The mitochondria powering your every heartbeat were, two billion years ago, free-living bacteria — and one day one of them moved in for good.

A cell inside a cell

Every complex living thing — you, an oak tree, a mushroom — is built from cells, and inside each of those cells live even smaller parts that were once independent bacteria. The mitochondrion, the tiny battery that turns your food and the air you breathe into usable energy, was one of them. So was the chloroplast, the green machine that lets a leaf eat sunlight.

The idea is that a big cell once swallowed a small bacterium and, instead of digesting it, kept it alive as a lodger. Over time the two became inseparable — one body, two genomes. Lynn Margulis argued that the most important cells on Earth are not single creatures at all, but old partnerships that never broke up.

The idea nobody wanted

When Lynn Sagan — she published under her married name before becoming known as Lynn Margulis — wrote this up in the mid-1960s, the notion sounded absurd to most biologists. An old, ridiculed idea, it was. She submitted the paper and, by her account, watched it bounce off about fifteen journals before the Journal of Theoretical Biology finally ran it in 1967.

Then it was mostly ignored — for nearly a decade. What rescued it was not rhetoric but evidence: once biologists could read DNA, they found that mitochondria and chloroplasts carry their own, and that it looks unmistakably bacterial. The heretic had been right.

Why it matters

Darwin had taught that new forms arise slowly, by small inherited changes sifted by survival. Margulis added a second, faster route to novelty: merger. Sometimes evolution does not tinker — it combines, fusing two whole organisms, and two whole genomes, into one. Without that single ancient merger there would be no animals, no plants, no fungi — no large life of any kind, because none of it could have harnessed oxygen the way mitochondria do.

The tenant who became family

Picture two companies that merge so completely they share one building, one payroll, one front door — yet each still keeps its own small filing cabinet of private records. The host cell is the building; the mitochondrion is the firm that moved in and stayed; and that little filing cabinet is the organelle's own DNA, the last proof that it once ran its own affairs. After a couple of billion years of living together, neither could survive being evicted.

A host cell you build up step by step — adding a former-bacterium mitochondrion, then a former-bacterium chloroplast — each drawn with its double membrane and its own loop of DNA.

Where it sits

Darwin (1859) gave life a single branching tree and a slow engine, natural selection; Avery and then Watson & Crick (1953) showed that heredity is written in DNA. Margulis added the twist that some of the branches on that tree did not just split — they grew back together and fused. Read alongside the Darwin and Watson–Crick entries in this Library, her paper completes a picture in which life advances both by dividing and, now and then, by joining.

The original document
Original source text
Lynn Sagan · Journal of Theoretical Biology · vol. 14, pp. 225–274 · 1967
Abstract
A theory of the origin of eukaryotic cells (“higher” cells which divide by classical mitosis) is presented. By hypothesis, three fundamental organelles: the mitochondria, the photosynthetic plastids and the (9+2) basal bodies of flagella were themselves once free-living (prokaryotic) cells.
The abstract then lays out the sequence: photosynthesis evolving under the anaerobic conditions of the early atmosphere; the later rise of aerobic metabolism during the transition to an oxidizing atmosphere; and, only afterward, the appearance of classical mitosis.
Classical mitosis evolved in protozoan-type cells millions of years after the evolution of photosynthesis.
The central proposal
An aerobic prokaryote is taken into the cytoplasm of a larger anaerobic host; the partnership becomes obligate, and the guest becomes the mitochondrion. A photosynthetic prokaryote, taken in later, becomes the plastid. Each acquisition is a separate symbiotic event — serial endosymbiosis.
The argument leaned on a discovery that fit it perfectly: mitochondria and plastids carry their own DNA, distinct from the nucleus and bacterial in character. The paper marshals comparative cytology, biochemistry and the geological record of atmospheric oxygen across some fifty pages.
[ … ]
Boston University · 1967