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Reading the Dose–Response Curve

Plot effect against dose and you get the most useful picture in pharmacology. Learn to read its height and its position separately, and EC50 and potency stop being jargon.

The shape of the curve

Give a tissue more and more of an agonist and measure the response each time. The picture you get is the dose–response curve. At very low doses almost nothing happens; in the middle the response climbs steeply; at high doses it flattens into a plateau because the receptors are running out. On a linear axis it looks like a lopsided hill. The standard fix is to plot dose on a logarithmic axis — the log dose plot — which stretches the low end and turns that lopsided hill into a clean, symmetric S-shaped (sigmoid) curve.

This is a graded response — measured in a single tissue or person, where the effect grows smoothly with dose. (Later you'll meet its cousin, the all-or-nothing quantal response across a population, when we discuss safety.) For now, the key skill is to read two features of this S-curve separately: how high it rises, and where along the dose axis it sits.

Height is efficacy; position is potency

The height of the plateau is the Emax — the maximum effect the drug can wring out of this system. It reflects efficacy from the last guide. A full agonist reaches a tall plateau; a partial agonist tops out lower no matter how much you give.

The position of the curve along the dose axis is captured by the EC50: the concentration producing 50% of that drug's own Emax. EC50 is the headline measure of potency — how *little* drug you need to get halfway. A curve sitting far to the left (small EC50) is a potent drug; one shoved to the right needs bigger doses for the same effect.

A worked comparison

Two pain drugs, same target receptor:

            EC50       Emax (max pain relief)
Drug X      2 mg       90%
Drug Y      40 mg      90%

Reading it:
- Same Emax (90%)  -> same EFFICACY (equally good at best)
- Drug X EC50 lower -> MORE POTENT (needs 20x less to reach halfway)

So Drug X is more potent but NOT more effective.
A lower-potency drug given at a bigger dose can do exactly the same job.
Same Emax, different EC50: equal efficacy, unequal potency.

When you compare two drugs on the same log-dose plot, vertical differences are about efficacy (Emax) and horizontal differences are about potency (EC50). Train your eye to look up-and-down versus left-and-right, and the whole curve becomes readable at a glance.